Products -> SGMDisc™ for Hydrogen Peroxide Vapor Biological Indicators

SGMDisc for hydrogen peroxide vapor contains bacterial spores on a stainless steel disc packaged within a convenient, peel-open Tyvek®/Mylar® envelope.

To use an SGMDisc, place the envelope inside the isolator and process normally. Upon removal from the isolator, send the envelope to the microbiology laboratory where the SGMDisc is to be asceptically transferred to soybean casein digest culture medium and incubated at the appropriate temperature for 7 days.

Availability

Use

Please follow instructions provided with each bag of HPV indicators

1. Place the HPV indicators in the most difficult to sterilize locations within the isolator which should include locations in and around equipment inside the isolator.
2. A minimum of ten (10) units per test is commonly used.
3. Expose the load to the sterilization and aeration cycle according to your documented procedures.
4. Following aeration, remove the HPV discs from the isolator using aseptic transfer techniques. Transfer the spore strips to the laboratory for culture.
5. In the laboratory:
   Using strict aseptic techniques and working in a Class 100 certified work station, transfer each biological indicator to an appropriate tube of culture medium (Soybean casein digest broth). Gently swirl the culture tube to assure wetting of the surfaces.
6. Incubate the discs at the appropriate temperature for seven days at 55-60°C.
7. If the spores survive the sterilization cycle, the culture medium will become turbid and cloudy. NOTE: Verify all positive cultures microscopically for gram positive, spore forming rods. Biochemical characterization is not necessary.
8. Record test results.
9. Autoclave all positive samples prior to disposal.

Storage

Room temperature.

Expiration

18 months from date of manufacture.

Certification

SGMDisc biological indicators are traceable to a recognized culture collection and certified for population and D-value.