DaAn Gene

About Daan Gene Co Ltd

Daan Gene Co Ltd, formerly DA AN GENE CO., LTD. OF SUN YAT-SEN UNIVERSITY, is a China-based company mainly engaged in the development, manufacture and distribution of reagents and instruments for clinical tests. The company’s main products include quantitative fluorescence polymerase chain reaction (PCR) series, public health series, time-resolved series, and enzyme-linked immunization series. The Company also provides independent clinical laboratory testing services. The Company operates its business in the domestic market.

Abstract

The current COVID-19 pandemic poses a threat to the world’s population with more than 21 million people infected. There is an urgent need to develop rapid and mass screening tools, as well as to identify and isolate infected people. We seek to evaluate different RT-qPCR kits and protocols to assess the best approach to use by skipping an RNA extraction step.

We have investigated the sensitivity and performance of different commercially available RT-qPCR kits to detect SARS-CoV-2 using 80 extracted RNAs and NSS from patients diagnosed with COVID-19. We evaluated the ability of each kit to detect viral RNA both from the extracted kit and directly from a previously boiled NSS, noting that direct RNA detection is possible when Ct values ​​are less than 30 with the three kits tested.

Since testing for SARS-CoV-2 in most places occurs once COVID-19 symptoms are apparent and therefore viral loads are expected to be high, our protocol will be helpful in supporting the SARS-CoV-2 diagnosis, especially in the United States where COVID-19 cases have skyrocketed in recent weeks, as well as in low- and middle-income countries, which would not have massive access to kit-based diagnosis. The information provided in this work paves the way for the development of more efficient SARS-CoV-2 detection approaches by avoiding an RNA extraction step.

Keywords: SARS-CoV-2, 2019-nCoV, COVID-19, RNA extraction, RT-PCR

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